ABSTRACT
Objective To establish a method for testing the dissolution of valsartan and metolazone tablets, and validate the method to provide a basis for drug quality control. Methods The dissolution was analyzed by HPLC method. An Agilent SB C18 (4.6 mm×250 mm, 5 μm) column and a mixture of acetonitrile and water were used with the ratio of 50: 50 at a flow rate of 1.0 ml/min with UV detection at 235 nm. Results The calibration curve of valsartan and metolazone was 6-120 μg/ml (r=1.0000, n=6) and 37.5-750 ng/ml (r=1.0000, n=6), respectively. The average recovery of the two drugs was 99.85% and 99.44% with RSD of 1.05% and 1.16% (n=9), respectively. The test solution was stable within 24 h and the filter did not interfere with determination as the two drugs were not adsorbed on it. RSD of this method were all less than 2%. Conclusion The method is simple, sensitive, accurate, and with highly distinguished ability for quality control.
ABSTRACT
Objective：To investigate the promotive effect of dendritic cells(DCs) on proliferation of CRTH2 (CD4(+)CD294(+)Th2) cells and the influence of CRTH2 cells on secretion of immunoglobulin from B cells so as to provide a new approach for amplification and sorting of Th2 cells. Methods：DCs were induced from peripheral blood mononuclear cells, then the loaded-BCGV-Ag-DCs were cocultured with T cells, and the mixed lymphocyte reaction(MLR) was performed by CCK8 method. The phenotypes of DCs and CRTH2 cells were detected by flow cytometry. CRTH2 cells sorted by MACS were co-cultured with B cells for 5 days to detect the secretion of immunoglobulin. Results：The subsets and absolute number CRTH2 cells were significantly increased by loaded-BCGV-Ag-DCs. The levels of IgG, IgA and IgE were higher increased in supernatant of CRTH2 and B cell co-culture system than that in control group or that in transwell group(P<0.05). Conclusion：The proliferation of CRTH2 cells can be greatly promoted by loaded-BCGV-Ag-DCs, and the CRTH2 cells can help B cells to secrete IgG, IgA and IgE.
Subject(s)
Humans , B-Lymphocytes , Cell Proliferation , Coculture Techniques , Dendritic Cells , Flow Cytometry , Immunoglobulins , Lymphocyte Culture Test, Mixed , Th2 CellsABSTRACT
<p><b>OBJECTIVE</b>To identify the mutation of ENG and ALK1 genes in a hereditary hemorrhagic telangiectasia pedigree.</p><p><b>METHODS</b>14 exons of ENG gene and 9 exons of ALK1 gene in 11 menbers of this pedigree 4 generation were amplified by reverse transcription-polymerase chain reaction (RT-PCR), the PCR products were screened by direct sequencing.</p><p><b>RESULTS</b>A nonsense mutation c.447G > A was found in exon 4 of ENG gen of the pedigreee, resulting in change of Trp 149 into Stop, while no gene mutation was found in ALK1 gene.</p><p><b>CONCLUSION</b>The hereditary hemorrhagic telangiectasia in this pedigree is caused by the nonsense mutation c.447G > A in ENG gene.</p>
Subject(s)
Humans , Codon, Nonsense , Exons , Mutation , Pedigree , Polymerase Chain Reaction , Telangiectasia, Hereditary HemorrhagicABSTRACT
<p><b>OBJECTIVE</b>To evaluate the efficacy of abdominal acupuncture for adhesion-stage shoulder periarthritis.</p><p><b>METHODS</b>One hundred and fifty-seven cases of shoulder periarthritis were randomly divided into an abdominal acupuncture group (79 cases) and a body acupuncture group (78 cases). The abdominal acupuncture was applied at Zhongwan (CV 12), Shangqu (KI 17) and Huaroumen (ST 24) in the abdominal acupuncture group while conventional acupuncture was applied at Jianyu (LI 15), Jianliao (TE 14) and Jianzhen (SI 9) in the body acupuncture group. The treatment was given three times a week for both groups and ten times made an observation course. Before and after treatment, visual analogue scale (VAS) was adopted for pain assessment and functional activity score (Mallet score) was used for shoulder joint function assessment for all the patients. Also the efficacy of both groups was compared.</p><p><b>RESULTS</b>The total effective rate in the abdominal acupuncture group was 92.4% (73/79), which was superior to 71.8% (56/78) in the body acupuncture group. The score of VAS after the treatment was 2.58 +/- 1.64 in the abdominal acupuncture group while 3.12 +/- 1.93 in the body acupuncture group, which had no statistical significance between each other (P > 0.05). The functional activity score after the treatment was 8.34 +/- 3.02 in the abdominal acupuncture group while 7.49 +/- 3.36 in the body acupuncture group, which had no statistical significance between each other (P > 0.05).</p><p><b>CONCLUSION</b>The abdominal acupuncture is an ideal treatment for adhesion-stage shoulder periarthritis, which has better total efficacy than conventional acupuncture. It achieves the same effect in relieving pain and improving functional activity as conventional acupuncture, but also has an advantage at fast selection of acupoint and less discomfort of needling sensation.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Acupuncture Points , Acupuncture Therapy , Periarthritis , Therapeutics , ShoulderABSTRACT
<p><b>OBJECTIVE</b>To compare therapeutic effects of elongated needle therapy and routine acupuncture therapy on dysuria induced by benign prostatic hyperplasia (BPH).</p><p><b>METHODS</b>Randomized, controlled, multi-central method was adopted and 150 cases confirmed to the enrolled criteria were divided into two groups by odd or even number, an elongated needle group (n = 72) and a routine acupuncture group (n = 78). Acupuncture was given at bilateral Zhibian (BL 54) and Zhongji (CV 3) in the two groups, once daily, 5 sessions constituting one course, with a 2-day interval between two courses. The treatment was given for 2 courses. Changes of I-PSS symptom cumulative score, urine flowing rate, residual urine in bladder before and after the treatment were observed.</p><p><b>RESULTS</b>The effective rate was 83.3% in the elongated needle group and 44.9% in the routine acupuncture group. There were significant differences between the two groups in improvemet of I-PSS score, increase of urine flowing rate and reduction of residual urine in bladder (all P < 0.05).</p><p><b>CONCLUSION</b>The elongated needle therapy has a definite therapeutic effect on dysuria induced by benign prostatic hyperplasia.</p>